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• epT.I.P.S.　LoRetention pipette tip and ep Dualfilter T.I.P.S.　LoRetention filter tips are ideal for use with samples containing surfactants. Liquids containing surfactants reduce surface tension. This causes the chip surface to become wet: liquid remains on the chip wall and cannot be collected. A significant amount of valuable samples have been found in the invisible coating left on the standard chip, which has also been discarded. Especially for sensitive PCR and real-timePCR applications, low adsorption surfaces can improve reproducibility and significantly reduce the loss of expensive reagents.
• epT.I.P.S.　Low adsorption characteristics of LoRetention pipette tip and ep Dualfilter T.I.P.S LoRetention filter tip is formed by superhydrophobic and extremely homogeneous surface. through innovative processing at the molecular level. We call this technology "Pearl Effect" technology. All pipette tips are uncoated, free of additives, and do not elute into the sample. Because the liquid containing the surfactant can be easily discharged, no liquid remains, especially on the chip. These superior properties ensure cost savings and improved reproducibility through the highest levels of sample recovery.
• Extremely homogeneous surface for maximum reproducibility
• Superhydrophobic surface that minimizes sample loss
• Significantly reduces bubble formation during pipetting
• Extremely high chemical resistance
• Selectable from PCR clean and Eppendorf Quality
• PCR clean/Sterile purity ep DualfilterT.I.P.S.　Also available (Sterilized, Pyrogen-Free)
• No coating
• Lot by lot test transcripts are available at: www.eppendorf.com/certificates .
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• Maximum reproducibility in genomics :
• epT.I.P.S.　LoRetention is especially recommended for applications such as PCR and real-timePCR, where maximum analytical accuracy for DNA/RNA analysis is required. For example, expensive master mixes and enzyme solutions tend to remain on the inner wall of the chip. Specially treated, low adsorptive surfaces have been developed to maximize surfactant solution bounce and handle valuable samples with minimal loss.
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• Example of a liquid in which loss occurs due to a surfactant :
• Master mix
• Enzyme solution: Restriction enzyme
• Ligation
• DNase
• DNA ladder for gel electrophoresis
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• epT.I.P.S.　As shown in Figure 1, LoRetention significantly outperforms the standard chip in terms of accuracy and sample recovery.
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• Maximum reproducibility in proteomics :
• In addition to molecular biology, highly sensitive detection methods require extreme reliability and reproducibility of pipette operation. In protein analysis and purification, reagents and samples often contain surfactants such as SDS. epT.I.P.S. by minimizing sample adsorption and improving the repeatability of pipette operation.　LoRetention chip and ep Dualfilter T.I.P.S.　LoRetention filter chips are also particularly useful for proteomics applications.
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• Protein-related applications :
• Separation
• Purification
• Denaturation
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• See actual results - compare to standard chip epT.I.P.S.　LoRetention performed remarkably well in terms of accuracy and sample recovery. As shown in Figures 1 and 2.