Spec

• Notes on usage: General Lab Use
• Brand name: Fast-Trap
• Main application: Lentivirus Purification and Concentration
• Store conditions: Fast-Trap Lentivirus purification and concentration kit should be kept at room temperature. Cautions and precautions for use: Cautions: This kitPurifies rapidly lentivirus which is an infectious agent to be treated based on the safety measures of biosafety level 2. In using this kit, wear personal protective equipment (PPE) to protect your hands, eyes, face, and body from the danger. Millipore CorporationIs not responsible for the improper use of this kit. Cautions: All steps of virus purification (except incubation and centrifugal separation in water bath), Should carried out in the biosafety cabinet. This product is for R&D use only. It cannot be used for medical diagnosis purpose nor clinical diagnosis purpose of humans. Lentivirus amount purified by this kitCan fluctuate depending on the success of the infection and the status of a cell.
• Package: Purification, concentration, 3 batches
• Overview: Fast Trap lentivirus purification and concentration kit
• Components: 3 - Fast-Trap virus filter unit; 3 - Steriflip-HV filter unit; 1 - 10 x connecting buffer (20mL); 1 - equilibrating buffer (100mL); 1 - cleaning buffer (200mL); 1 - elution buffer (21mL); 3 - Amicon Ultra-4 filter unit (100kDa, NMWL); 1 - test tube stand; 1 - user guide; 1 - workflow overview card; 1 - safety data sheet about such as chemical material
• Product overview: In Fast Trap lentivirus purification and concentration kit, Fast Trap adenovirus purification and concentration kit includes reagent needed to purify virus for the expression of recombinant gene from contaminants in the cell, filtration device, and concentration device. Concentrated virus can be gained in an arbitrary replacement buffer suited to an In vitro test and an animal experimentation. The kit can purify 3mL or more of lentivirus particle with infectious ability, 2 x 10 to 8 pieces at its maximum, in laboratory-scale purification, using a rapid and simple membrane method. In purification kit protocol, it collects culture medium and supernatant of the infected cell-released virusMost of the large residue isRemoved by centrifugation separation method, An infectable virus particle remains in the supernatant. The supernatant is filtered by a Steriflip(R) -HV 0.45μm filter for further clarification. After adding a connecting buffer, Filter virus solution with a virus purification filter, Absorb virus particle. At this time, the smaller cell residue and medium are passed through a filter. After cleaning to remove weak binding protein, Elute the virus from the filter by an elution bufferLastly, By using Amicon(R) Ultra centrifugation filter unit, Concentrate the virus, Change to a desired buffer. Precaution for use: Unless otherwise specified clearly in the catalog of our products or the documentations that are attached with the product, Our products are for research application purposes only, Any other purpose to use (including without limitation the use for commercial purposes without permission, the use for in vitro diagnosis, the use for ex vivo or in vivo therapy, or any use and application to human or animal) is not permitted. Main application: Precautions about purification and concentration of lentivirus: Intended only for the use in a common laboratory.
• ※We ask for your kind understanding that lead time might change If it is out of stock in Japan.
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