Features

• For stepping up your first self-inspection or inspection!
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• inspection procedure
• 【I】specimen pretreatment
• ① We collect the food (sample) to be tested. The instruments used for sampling (tweezers, scissors) should be sterilized or sterilized by alcohol or flame sterilization.
• ② Set the weighing processing sample collection bag stand on the balance. Insert the Stomac bag (E-Mix Homogenize bag) into the balance bag stand and open it. Tare the balance and weigh 10 g of the sample into a Stomach bag (instruments must be sterilized at this time). Then add sterile diluted water OR-90 (90 mL) to make a 10 fold diluted sample solution.
• ③ Mix a Stomac bag containing 10 g of the sample + sterile diluted water OR-90 (90 mL) with a Stomacker (bag mixer E-Mix).
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• 【II】serial dilution
• ④ Dilute further using 10 times dilution. Place the treated Stomac bag on the food sample stand as shown in the photo and use a micropipette to aspirate 1 mL.
• ⑤ Inject 1 mL of the aspirated 10 × diluted sample solution into sterile diluted water OR-9 (9 mL) to complete the 100 × diluted sample solution (Repeat as shown in the figure if necessary to make 1000 or 10,000 times diluted sample solution.).
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• [III] Sample inoculation
• ⑥ Drop 1 mL of the 10, 100 and 1000 times diluted sample solution onto 2 sheets of Petri FilmTM, and cover with the film.
• ⑦ Press lightly with a special tool (spreader) and mold into a circle.
• ⑧ Once it has solidified into a gel, put it in an incubator and incubate at the specified temperature and time. *Petri FilmTM for coliform bacteria and E. coli is difficult to determine after incubation if air enters the film. Please be careful.
• ⑨ After incubation, measure with a colony counter pen. 30~300 colonies is an appropriate number. The average value is obtained for each dilution and the number of bacteria is calculated by multiplying the dilution ratio.
• ⑩ Put the used Petri FilmTM in a sterilization bag, sterilize by high pressure steam, and discard.
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• Bacterial count calculation method [Example]
• ① Average number of colonies (155+180) ÷2=167.5≒168 in 2 sheets of 10 × dilution solution
• ② Mean number of colonies multiplied by dilution factor: 168 x 10=1680
• ③ Multiplier: 1680=1.68 x 10^3
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• Bacterial count: 1.7×10^3 CFU/mL
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• When the dilution ratio is 10: 1 sheet/155, 2 sheets/180
• When the dilution ratio is 100: 1 sheet/20, 2 sheets/17
• The basic equipment necessary for food microorganism inspection is summarized.